EMBOSS: patmatdb

patmatdb

Function

Search a protein sequence with a motif

Description

Takes a protein motif and compares it to a set of protein sequences.

It returns the number of matches there were between the motif and each matched sequence, length of match, start and end positions of match, and writes out an alignment.

Usage

Here is a sample session with patmatdb

% patmatdb Search a protein sequence with a motif Input protein sequence(s): tsw:* Protein motif to search for: st[ty]s Output report [edd1_rat.patmatdb]:

Go to the input files for this example
Go to the output files for this example

Command line arguments

   Standard (Mandatory) qualifiers:
  [-sequence]          seqall     Protein sequence(s) filename and optional
                                  format, or reference (input USA)
  [-motif]             string     Patterns for patmatdb are based on the
                                  format of pattern used in the PROSITE
                                  database. For example:
                                  '[DE](2)HS{P}X(2)PX(2,4)C' means two Asps or
                                  Glus in any order followed by His, Ser, any
                                  residue other then Pro, then two of any
                                  residue followed by Pro followed by two to
                                  four of any residue followed by Cys. The
                                  search is case-independent, so 'AAA' matches
                                  'aaa'. (Any string of at least 2
                                  characters)
  [-outfile]           report     [*.patmatdb] Output report file name

   Additional (Optional) qualifiers: (none)
   Advanced (Unprompted) qualifiers: (none)
   Associated qualifiers:

   "-sequence" associated qualifiers
   -sbegin1            integer    Start of each sequence to be used
   -send1              integer    End of each sequence to be used
   -sreverse1          boolean    Reverse (if DNA)
   -sask1              boolean    Ask for begin/end/reverse
   -snucleotide1       boolean    Sequence is nucleotide
   -sprotein1          boolean    Sequence is protein
   -slower1            boolean    Make lower case
   -supper1            boolean    Make upper case
   -sformat1           string     Input sequence format
   -sdbname1           string     Database name
   -sid1               string     Entryname
   -ufo1               string     UFO features
   -fformat1           string     Features format
   -fopenfile1         string     Features file name

   "-outfile" associated qualifiers
   -rformat3           string     Report format
   -rname3             string     Base file name
   -rextension3        string     File name extension
   -rdirectory3        string     Output directory
   -raccshow3          boolean    Show accession number in the report
   -rdesshow3          boolean    Show description in the report
   -rscoreshow3        boolean    Show the score in the report
   -rusashow3          boolean    Show the full USA in the report
   -rmaxall3           integer    Maximum total hits to report
   -rmaxseq3           integer    Maximum hits to report for one sequence

   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write standard output
   -filter             boolean    Read standard input, write standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages

Standard (Mandatory) qualifiers Allowed values Default

[-sequence]
(Parameter 1) Protein sequence(s) filename and optional format, or reference (input USA) Readable sequence(s) Required

[-motif]
(Parameter 2) Patterns for patmatdb are based on the format of pattern used in the PROSITE database. For example: '[DE](2)HS{P}X(2)PX(2,4)C' means two Asps or Glus in any order followed by His, Ser, any residue other then Pro, then two of any residue followed by Pro followed by two to four of any residue followed by Cys. The search is case-independent, so 'AAA' matches 'aaa'. Any string of at least 2 characters Required

[-outfile]
(Parameter 3) Output report file name Report output file <*>.patmatdb

Additional (Optional) qualifiers Allowed values Default

(none)

Advanced (Unprompted) qualifiers Allowed values Default

(none)

Standard (Mandatory) qualifiers	Allowed values	Default
[-sequence] (Parameter 1)	Protein sequence(s) filename and optional format, or reference (input USA)	Readable sequence(s)	Required
[-motif] (Parameter 2)	Patterns for patmatdb are based on the format of pattern used in the PROSITE database. For example: '[DE](2)HS{P}X(2)PX(2,4)C' means two Asps or Glus in any order followed by His, Ser, any residue other then Pro, then two of any residue followed by Pro followed by two to four of any residue followed by Cys. The search is case-independent, so 'AAA' matches 'aaa'.	Any string of at least 2 characters	Required
[-outfile] (Parameter 3)	Output report file name	Report output file	<>*.patmatdb
Additional (Optional) qualifiers	Allowed values	Default
(none)
Advanced (Unprompted) qualifiers	Allowed values	Default
(none)

Input file format

patmatdb reads in one or more protein sequence USAs.

Input files for usage example

'tsw:*' is a sequence entry in the example protein database 'tsw'

Pattern specification

Patterns for patmatdb are based on the format of pattern used in the PROSITE database, with the difference that the terminating dot '.' and the hyphens, '-', between the characters are optional.

The PROSITE pattern definition from the PROSITE documentation follows.

The standard IUPAC one-letter codes for the amino acids are used.
The symbol `x' is used for a position where any amino acid is accepted.
Ambiguities are indicated by listing the acceptable amino acids for a given position, between square parentheses `[ ]'. For example: [ALT] stands for Ala or Leu or Thr.
Ambiguities are also indicated by listing between a pair of curly brackets `{ }' the amino acids that are not accepted at a given position. For example: {AM} stands for any amino acid except Ala and Met.
Each element in a pattern is separated from its neighbor by a `-'. (Optional in patmatdb).
Repetition of an element of the pattern can be indicated by following that element with a numerical value or a numerical range between parenthesis. Examples: x(3) corresponds to x-x-x, x(2,4) corresponds to x-x or x-x-x or x-x-x-x.
When a pattern is restricted to either the N- or C-terminal of a sequence, that pattern either starts with a `<' symbol or respectively ends with a `>' symbol.
A period ends the pattern. (Optional in patmatdb).

For example, in SWISSPROT entry 100K_RAT you can look for the pattern:

[DE](2)HS{P}X(2)PX(2,4)C

This means: Two Asps or Glus in any order followed by His, Ser, any residue other then Pro, then two of any residue followed by Pro followed by two to four of any residue followed by Cys.

The search is case-independent, so 'AAA' matches 'aaa'.

Output file format

The output is a standard EMBOSS report file.

The results can be output in one of several styles by using the command-line qualifier -rformat xxx, where 'xxx' is replaced by the name of the required format. The available format names are: embl, genbank, gff, pir, swiss, trace, listfile, dbmotif, diffseq, excel, feattable, motif, regions, seqtable, simple, srs, table, tagseq

See: http://emboss.sf.net/docs/themes/ReportFormats.html for further information on report formats.

By default patmatdb writes a 'dbmotif' report file.

Output files for usage example

File: edd1_rat.patmatdb

########################################
# Program: patmatdb
# Rundate: Sun 15 Jul 2007 12:00:00
# Commandline: patmatdb
#    -sequence "tsw:*"
#    -motif "st[ty]s"
# Report_format: dbmotif
# Report_file: edd1_rat.patmatdb
########################################

#=======================================
#
# Sequence: HD_FUGRU     from: 1   to: 3148
# HitCount: 1
#
# Motif: ST[TY]S
#
#=======================================

Length = 4
Start = position 1038 of sequence
End = position 1041 of sequence


STPASSTTSSAVDP
     |  |
  1038  1041


#---------------------------------------
#---------------------------------------
#=======================================
#
# Sequence: GCN4_YEAST     from: 1   to: 281
# HitCount: 1
#
# Motif: ST[TY]S
#
#=======================================

Length = 4
Start = position 158 of sequence
End = position 161 of sequence


SNLEVSTTSFLPTP
     |  |
   158  161


#---------------------------------------
#---------------------------------------
#=======================================
#
# Sequence: PAXI_HUMAN     from: 1   to: 591
# HitCount: 1
#
# Motif: ST[TY]S
#
#=======================================

Length = 4
Start = position 13 of sequence
End = position 16 of sequence


LADLESTTSHISKR
     |  |
    13  16


#---------------------------------------
#---------------------------------------

#---------------------------------------
# Total_sequences: 3
# Total_hitcount: 3
#---------------------------------------

Data files

None.

Notes

None.

References

Bairoch, A., Bucher P. (1994) PROSITE: recent developments. Nucleic Acids Research, Vol 22, No.17 3583-3589.
Bairoch, A., (1992) PROSITE: a dictionary of sites and patterns in proteins. Nucleic Acids Research, Vol 20, Supplement, 2013-2018.
Peek, J., O'Reilly, T., Loukides, M., (1997) Unix Power Tools, 2nd Edition.
Gusfield, D., (1997) Algorithms on strings, Trees and Sequences.
Sedgewick, R,. (1990) Algorithms in C.

Warnings

None.

Diagnostic Error Messages

None.

Exit status

It always exits with status 0

Known bugs

None.

Program name	Description
antigenic	Finds antigenic sites in proteins
digest	Protein proteolytic enzyme or reagent cleavage digest
epestfind	Finds PEST motifs as potential proteolytic cleavage sites
fuzzpro	Protein pattern search
fuzztran	Protein pattern search after translation
helixturnhelix	Report nucleic acid binding motifs
oddcomp	Find protein sequence regions with a biased composition
patmatmotifs	Search a PROSITE motif database with a protein sequence
pepcoil	Predicts coiled coil regions
preg	Regular expression search of a protein sequence
pscan	Scans proteins using PRINTS
sigcleave	Reports protein signal cleavage sites

Author(s)

Sinead O'Leary (current e-mail address unknown)
while she was at:
HGMP-RC, Genome Campus, Hinxton, Cambridge CB10 1SB, UK

History

Completed April 30 1999.

Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

Comments

None

Function

Description

Usage

Command line arguments

Input file format

Input files for usage example

Pattern specification

Output file format

Output files for usage example

File: edd1_rat.patmatdb

Data files

Notes

References

Warnings

Diagnostic Error Messages

Exit status

Known bugs

See also

Author(s)

History

Target users

Comments